Fig. 1: The expressions of Nrf2, Numb and EMT in BLM-induced PF.

C57 mice were treated with an endotracheal injection of BLM (4.5 mg/kg) for 7, 14 and 28 days. a The lung sections from each mouse were stained with H&E staining and Masson’s trichrome staining to observe the histopathological structure and collagen accumulation respectively. Scale bars represent 100 μm. (The black arrows indicated the collagen expression). b Relative protein expressions of Nrf2, Numb, α-SMA and E-cad were measured by IHC. Scale bars represent 50 μm. Boxes were used to enlarge positive tissue, which expressed target proteins