Fig. 2: miR-195 delays cell cycle progression at G1 and induces apoptosis and senescence in NSCLC cells.
From: miR-195 targets cyclin D3 and survivin to modulate the tumorigenesis of non-small cell lung cancer
a Cell cycle distribution as a function of miR-195 transfection in NSCLC cells. Fractions of cells in G1, S, and G2 phases were estimated using the Watson pragmatic model. b Time-dependence of induction of apoptosis by miR-195 in NSCLC cells. c PARP and cleaved PARP protein levels in NSCLC cells transfected with miR-195 mimic and inhibitors. d Cell confluence as a function of time after miR-195 transfection in NSCLC cells, quantified using the IncuCyte FLR live cell imaging system. e β-gal staining of cells transfected with miR-195 mimic or BIRC5 siRNAs. f Quantification of β-gal staining. g p53 and p21 protein levels in NSCLC cells transfected with control or miR-195 oligos. h Rb and p-Rb protein levels in NSCLC cells transfected with control or miR-195 oligos. i Rb and p-Rb protein levels in NSCLC cells after BIRC5 knockdown. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001
