Fig. 5: OPN promotes ICC progression via interacting with β-Catenin and activating Wnt signaling. | Cell Death & Disease

Fig. 5: OPN promotes ICC progression via interacting with β-Catenin and activating Wnt signaling.

From: Osteopontin promotes metastasis of intrahepatic cholangiocarcinoma through recruiting MAPK1 and mediating Ser675 phosphorylation of β-Catenin

Fig. 5

a Down-regulation of OPN inhibited the Wnt/β-Catenin pathway, while β-Catenin overexpression could rescue the expression of cyclin-D1, c-Myc, and Prox1 caused by OPN knockdown. b, c Knockdown of OPN significantly suppressed the proliferation (b) and invasion abilities (c) of CCLP1 cells, which was counteracted by β-Catenin overexpression. d RBE cells (5 × 105) stably expressing empty vector, Flag-OPN, or Flag-OPN plus shβ-Catenin were injected subcutaneously into nude mice. Tumor volumes of subcutaneous implantation models of ICC were shown. e, f OPN promotes ICC metastasis in vivo. Nude mice were implanted with RBE stable cell lines described as above into the intraperitoneal cavity; the mesenteric lymph and lung metastatic nodes were counted under a microscope

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