Fig. 2: B4GalT5 knockdown in subcutaneous adipose tissue prevents obesity-induced insulin resistance and adipose inflammation. | Cell Death & Disease

Fig. 2: B4GalT5 knockdown in subcutaneous adipose tissue prevents obesity-induced insulin resistance and adipose inflammation.

From: Downregulation of β1,4-galactosyltransferase 5 improves insulin resistance by promoting adipocyte commitment and reducing inflammation

Fig. 2

Six-week-old C57BL/6 J mice and 4-week-old ob/ob mice were injected with adenovirus expressing B4GalT5 or control LacZ shRNA twice a week s.c. adjacent to both sides of the inguinal fat pads for 6 weeks. WT groups of mice were fed with HFD, ob/ob mice were fed with ND. a–b Blood glucose was measured under fasting and fed conditions (n = 6/group). c–d GTT and ITT assays were conducted in HFD mice (n = 6/group) and ob/ob mice (n = 6/group). e Western blotting analysis of the insulin-signaling pathways in HFD-fed mice and ob/ob mice treated with B4GalT5 or LacZ shRNA adenovirus. These mice were killed after insulin stimulation (0.75 mU/g body weight) for 5 min via i.p. f–g qPCR analysis of the mRNA level of proinflammatory cytokines in inguinal fat pads of HFD mice (n = 8/group) and ob/ob mice (n = 6/group). Statistical analysis was performed by collating the results from multiple samples. *P < 0.05, **P < 0.01, ***P < 0.001

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