Fig. 3: Downregulation of B4GalT5 increased adipogenesis in subcutaneous adipose tissue. | Cell Death & Disease

Fig. 3: Downregulation of B4GalT5 increased adipogenesis in subcutaneous adipose tissue.

From: Downregulation of β1,4-galactosyltransferase 5 improves insulin resistance by promoting adipocyte commitment and reducing inflammation

Fig. 3

Six-week-old C57BL/6 J mice were injected with adenovirus expressing B4GalT5 or control LacZ shRNA twice a week s.c. adjacent to both sides of the inguinal fat pads for 6 weeks. Both groups of mice were fed with HFD. a Knockdown of B4GalT5 expression in inguinal fat pads was confirmed by qPCR (n = 8/group). b IHC staining for B4GalT5 was conducted in the inguinal fat pads of mice treated with B4GalT5 or LacZ shRNA adenovirus (Scale bar: 20 μm). B4GalT5 IHC results were quantified by Image pro plus 6.0 image analysis software. c Comparison of inguinal fat pads in mice treated with B4GalT5 or LacZ shRNA adenovirus. Fat pads were weighed and statistically analyzed. d Representative H&E staining of inguinal fat pads in mice treated with B4GalT5 or LacZ shRNA adenovirus. Scale bar: 20 μm. Cell diameter was measured in the H&E-stained sections of three individual samples in each group. e Relative DNA amount of the whole inguinal fat pads from mice treated with B4GalT5 or LacZ shRNA (n = 4). f The percentage of mature adipocytes relative to total cell number in inguinal fat pads was statistically analyzed by flow cytometry between the two groups (n = 8). Statistical analysis was performed by collating the results from multiple samples. *P < 0.05, **P < 0.01, ***P < 0.001

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