Fig. 5: ELK1 directly binds to miR-30c and miR-21 proximal promoter regions. | Cell Death & Disease

Fig. 5: ELK1 directly binds to miR-30c and miR-21 proximal promoter regions.

From: KRAS induces lung tumorigenesis through microRNAs modulation

Fig. 5

a Schematic representation of ELK1-binding sites in miR-30c and miR-21 promoters. b, c ELK1 knockdown reduced miR-30c and miR-21 expression in A549 and Calu-1 cells. (Fig. 5b top panel, Calu-1 cells; bottom panel, A549 cells) d Schematic representation of ELK1-binding site deletions. e, f ELK1 silencing reduced luciferase activity whereas deletion of the binding sites rescued luciferase activity. g ChIP showing a direct interaction between ELK1 and miR-30c and miR-21 promoter regions. Bars indicate mean ± SD (n = 3) and the P values were addressed by two-tailed Student’s t test (*P < 0.05, **P < 0.001)

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