Fig. 6: MiR-30c and miR-21 promote tumorigenesis in vivo.
From: KRAS induces lung tumorigenesis through microRNAs modulation
a MiR-30c and miR-21 upregulation in tumor samples compared to normal lung (tumor samples n = 21; normal lung n = 21). b, c KRAS and ELK1 directly correlate with miR-30c in lung tumors (tumor samples n = 21). d Pearson correlation between Elk1 and miR-21 in 160 (155 KRASWT and 5 KRASG12D) adenocarcinoma samples. e, f MiR-30c (44 normal samples, 150 lung adenocarcinoma KRASWT, and 5 lung adenocarcinoma KRASG12D) and miR-21 (46 normal samples, 155 lung adenocarcinoma KRASWT, and 5 lung adenocarcinoma KRASG12D) expression in tumors expressing KRASWT and KRASG12D compared to normal samples from the TCGA data set LUAD. g MiR-30c and miR-21 are significantly upregulated in tumors from KRASG12D mice compared to control mice. h Representative pictures of the effect of LNA-anti-miR-21 (a–c) and LNA-anti-miRNA control (d–f) treatment 12 weeks post adenoCRE inhalation in KRASG12D transgenic mice. Lungs from mice treated with anti-miR-21 presented a normal alveolar texture with normal bronchiolar structures and no or minimal signs of fibrosis/flogosis (a). Normal representation of bronchiolar epithelial cells (b) and of alveolar framework (c) is shown. KRASG12D mice treated with anti-miR control developed the usual preneoplastic lesions observed in this model. A significant disarrangement of the pulmonary parenchyma (d), with the accumulation of preneoplastic lesions such as atypical adenomatous hyperplasias (e) or adenomas (f; the largest adenoma is showed). Scale bars 100 µm. i KI67 in lungs from mice treated with anti-miR-ctr and anti-miR-21. j, k qPCR showing miR-30c and miR-21 levels in plasma samples obtained from patients undergoing surgical resection of NSCLC. P peripheral vein, C cancer draining pulmonary vein, P1–P6 patient 1–6. l MiR-30c and miR-21 expression levels in plasma from KRASG12D mice treated with anti-miR-ctr or anti-miR-21. Bars indicate mean ± SD (n = 3) and the P values were addressed by two-tailed Student’s t test (*P < 0.05, **P < 0.001)
