Fig. 2: PANDAR promoted malignant progression of GC cells. | Cell Death & Disease

Fig. 2: PANDAR promoted malignant progression of GC cells.

From: Long noncoding RNA PANDAR blocks CDKN1A gene transcription by competitive interaction with p53 protein in gastric cancer

Fig. 2

a Northern blot analysis of PANDAR expression in normal gastric tissues and GC cell lines. b QRT-PCR analysis of PANDAR expression in normal gastric tissues and GC cell lines. c Quantitative methylation-specific PCR analysis of PANDAR promoter methylation levels in GC tissues and paired adjacent tissues. M, methylation-specific primer; U, unmethylation-specific primer. d Cell viability was measured by the CCK-8 assay at 1, 3, 5 and 7 days. e Colony formation was scored after 2 weeks. The number of colonies in the graphs was representative of three independent experiments (lower panel). f The effect of PANDAR knockout on the cell-cycle progression of AGS cells. g The effect of PANDAR knockout on sensitizing AGS cells to apoptosis. h Cell viability was measured by the CCK-8 assay at 1, 3, 5 and 7 days. i Colony formation was scored after 2 weeks. The number of colonies in the graphs was representative of three independent experiments (lower panel). j The effect of PANDAR overexpression on the cell-cycle progression of SNU-1 cells. k The effect of PANDAR overexpression on inhibiting SNU-1 cells to apoptosis. (*p < 0.05; **p < 0.01; ***p < 0.001)

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