Fig. 5: PANDAR directly interacted with p53 protein. | Cell Death & Disease

Fig. 5: PANDAR directly interacted with p53 protein.

From: Long noncoding RNA PANDAR blocks CDKN1A gene transcription by competitive interaction with p53 protein in gastric cancer

Fig. 5

a RIP analysis determined the recovery of PANDAR in cancer cells using p53 antibody. b RNA pull-down analysis determined the p53 protein−PANDAR interaction using 5′ biotin-linked RNAs. U6 RNA as a negative control. c RIP analysis determined the recovery of a portion of PANDAR in AGS cells using p53 antibody. d Alignment and conserved putative p53 binding sites in CDKN1A promoter and PANDAR nucleic acid sequence. e RNA pull-down analysis determined the p53 protein−PANDAR interaction using 5’ biotin-linked RNAs. f Immunoblotting analysis determined the levels of p21 protein in cancer cells treated with different expression vectors. g QRT-PCR analysis determined the expression of CDKN1A mRNA in cancer cells treated with different expression vectors. h Immunoblotting analysis determined the p53 protein levels using the p53 antibody and HA antibody. i RIP analysis determined the recovery of PANDAR in KATOIII cells transfected with different mutated-P53 gene expression vectors using HA antibody. j RNA pull-down analysis determined the PANDAR-p53 protein interaction using protein lysis of KATOIII cells transfected with different mutated-TP53 gene expression vectors

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