Fig. 4: Tan IIA did not inhibit Hsp90. | Cell Death & Disease

Fig. 4: Tan IIA did not inhibit Hsp90.

From: The antitumor natural product tanshinone IIA inhibits protein kinase C and acts synergistically with 17-AAG

Fig. 4

a The binding between Tan IIA (100 μM) and Hsp90 protein was examined by the thermal stabilization-based CETSA method at a range of temperatures. b The intensities of bands shown in (a) were quantified and the data are presented as mean ± S.D. of three independent experiments. c Western blot analysis of Hsp70 protein and Hsp90 client proteins IKK and EGFR after 24 h Tan IIA treatment. The Hsp90 inhibitor 17-AAG (10 μM) was included as a positive control

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