Fig. 7: Tan IIA inhibited the PI3K/Akt/mTOR pathway and induced cell cycle arrest and autophagy in MCF7 cells. | Cell Death & Disease

Fig. 7: Tan IIA inhibited the PI3K/Akt/mTOR pathway and induced cell cycle arrest and autophagy in MCF7 cells.

From: The antitumor natural product tanshinone IIA inhibits protein kinase C and acts synergistically with 17-AAG

Fig. 7

a The activity of the PI3K/Akt/mTOR pathway in Tan IIA treated MCF-7 cells was monitored by measuring the levels of active PI3K (p-PI3K), Akt (p-Akt), and mTOR (p-mTOR), and the levels of total proteins for each kinase. b Flow cytometry-based analysis of cell cycle distribution in control and Tan IIA treated MCF-7 cells. c To determine the extent of autophagy, western blot analysis was performed to detect LC3-II proteins in lysate samples prepared from control and Tan IIA-treated MCF-7 cells. For comparison, western blot results of LC3-I and β-actin were also included

Back to article page