Fig. 6: Exogenous administration of NGB peptides boosts axon regeneration in neurons via p38 after OGD/Re. | Cell Death & Disease

Fig. 6: Exogenous administration of NGB peptides boosts axon regeneration in neurons via p38 after OGD/Re.

From: Neuroglobin boosts axon regeneration during ischemic reperfusion via p38 binding and activation depending on oxygen signal

Fig. 6

a, b Effects of NGB peptides on neurite/axon regeneration in N2a cells (a) or neurons (b) upon OGD/Re. TAT-His-NGB or His-NGB at 40 nM was supplemented during reoxygenation incubation. Representative micrographs and statistical analysis demonstrated that TAT-His-NGB but not His-NGB entered into neuronal cells and significantly increased neurite/axon length after OGD-1 h/Re-24 h. Data are presented as means ± S.E.M. **P < 0.01, N = 3. c Co-localization of NGB peptides and GAP43 in the growth cone of neuron upon OGD/Re. d Effects of NGB peptides on GAP43 and p-p38 expression. Results of western blotting analysis demonstrated that administration of TAT-His NGB induced p38 activation and upregulated GAP43. Data are presented as means ± S.E.M. **P < 0.01, N = 3. e Proposed role and mechanism of Ngb in axon regeneration after I/R. After reoxygenation, the binding of O2 in Ngb reinforces its p38 binding, which facilitates p38 activation and finally initiates axon regeneration. ↓ or ↑, increase or decrease; →, induction or activation; ↔, binding; ⊥, inhibition

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