Fig. 1: MET and SPRY2 expression varies across RMS cell lines. | Cell Death & Disease

Fig. 1: MET and SPRY2 expression varies across RMS cell lines.

From: SPRY2 is a novel MET interactor that regulates metastatic potential and differentiation in rhabdomyosarcoma

Fig. 1

Quantification of MET (a) and SPRY2 (b) transcripts in all five RMS cell lines was carried out by quantitative real-time PCR (qPCR). RD (ERMS) cells have lower levels of MET but higher levels of SPRY2 transcripts as compared to ARMS cell lines used in the study. GAPDH and HPRT were used for normalization. Representative immunoblots and densitometric analyses normalized to β-ACTIN show protein expression of MET (c, e) and SPRY2 (d, f) is variable in different RMS cell lines similar to the variability observed in their transcript levels (a and b, respectively). As with the transcript expression profiles, RD (ERMS) cells show lesser MET but higher SPRY2 protein expression compared to ARMS cell lines. Note that the Y axes of the densitometry graphs have variable scales owing to varying levels of expression. Immunofluorescence confocal imaging shows cytoplasmic localization of MET (red, left panels) and SPRY2 (red, right panels) in RD (g) and SJRH30 (h) cells, along with phalloidin labeling the actin fibers (green) and nuclear staining with DAPI (blue). Scale bar=25 µm

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