Fig. 4: Knockdown of SPRY2 significantly reduces RMS cell migratory potential recapitulating the effect of MET downregulation. | Cell Death & Disease

Fig. 4: Knockdown of SPRY2 significantly reduces RMS cell migratory potential recapitulating the effect of MET downregulation.

From: SPRY2 is a novel MET interactor that regulates metastatic potential and differentiation in rhabdomyosarcoma

Fig. 4

Representative bright field micrographs of wound closure assays on RD (a) and SJRH30 (c) RMS cells imaged at specific time points after ~96-h of transfection with MET or SPRY2 siRNAs, compared to those transfected with control siRNA. Scale bar is 100 µm. Bar graphs summarizing percent wound closure in the monolayers of RD (b) and SJRH30 (d) cells indicates that wound closure is significantly decreased in MET and SPRY2 siRNA transfected RMS cells when compared to control siRNA transfected cells. The graphical data represent the mean ± SEM of a minimum of 5 independent experiments. Representative western blots using cell lysates prepared at the end of the scratch assay show that MET (upper panels) and SPRY2 (middle panels) knockdown is maintained in RD (b′) and SJRH30 (d′) cells until after the completion of wound healing assay

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