Fig. 4: STK17B induces EMT process via activating AKT/GSK-3β/Snail pathway. | Cell Death & Disease

Fig. 4: STK17B induces EMT process via activating AKT/GSK-3β/Snail pathway.

From: STK17B promotes carcinogenesis and metastasis via AKT/GSK-3β/Snail signaling in hepatocellular carcinoma

Fig. 4

a The expression of STK17B, E-cadherin, N-cadherin, Vimentin, and Snail was measured by western blotting. GAPDH was used as internal control. b mRNA expression of Snail, Slug, Zeb-1, Zeb-2, and Twist was evaluated after overexpressing or silencing STK17B. c Snail knockdown significantly decreased STK17B-induced EMT, while Snail overexpression enhanced it. d Immunofluorescence analysis of E-cadherin and vimentin expression in SMMC7721 and HCCLM3 cells. e Snail knockdown significantly decreased STK17B-enhanced migration and invasion, while Snail overexpression promoted shSTK17B-inhibited migration and invasion. f Representative images of lung metastasis nodules (left panel). Number of the metastasis nodules per lung (right panel). g Expression of certain key molecule in several signaling pathway was analyzed by western blotting. h Association between STK17B and AKT/GSK-3β/Snail signal was accessed by western blotting. All data are expressed as mean ± SD of three separate experiments. **P < 0.01; ***P < 0.001

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