Fig. 2

Loss of Ndrg2 influences tumor-associated macrophage polarization. a Flow cytometry analysis of macrophages infiltrated into WT or Ndrg2−/− mouse metastatic lesions. Among the CD45⁺ cells, BM-derived TAMs and Kupffer cells were gated and analyzed. n = 5−7 mice per group. b Flow cytometry analysis of macrophage subpopulations based on Ly6C and MHCII expression. Among F4/80⁺ macrophages, Ly6C^lo MHCII^hi M1-like TAMs and Ly6C^lo MHCII^lo M2-like TAMs were gated and analyzed. n = 6 mice per group. c At 14 days after metastasis model establishment, macrophages in WT or Ndrg2−/− mouse metastatic lesions were isolated using magnetic beads, and q-PCR was used to test the indicated markers. n = 3 mice per group. The results are presented as the mean ± SEM *p < 0.05; **p < 0.01