Fig. 5

Ndrg2 influences M1-like macrophage polarization in vitro. a Relative expression changes in Ndrg2 during the maturation process of monocytes toward macrophages. Monocytes/macrophages were isolated using magnetic beads and tested via q-PCR on days 0, 2, 4, and 6. b Relative expression of Ndrg2 in M0, M1, and M2 macrophages and TAMs determined by q-PCR. n = 3 per group. c, d Relative expression of M1 and M2 markers in WT or Ndrg2−/− M1 and M2 BMDMs determined by q-PCR. n = 3 per group. e Relative expression of M1 and M2 markers in WT or Ndrg2−/− TAMs (induced by CMT93 cell-conditioned medium) determined by q-PCR. n = 3 per group. f CD86 and CD206 expression in CMT93 cell-conditioned medium-induced WT or Ndrg2−/−TAMs was analyzed using flow cytometry. n = 3 per group. g IL-12 and IL-10 concentrations in WT or Ndrg2−/− M0, M1, and M2 macrophages and TAM culture medium tested via ELISA. n = 3 per group. The results are presented as the mean ± SEM *p < 0.05, **p < 0.01, and ***p < 0.001