Fig. 6
Loss of Ndrg2 influences macrophage polarization through the activation of NF-κB signaling. a WT or Ndrg2−/− BMDMs were treated with 20 ng/ml TNF-α for the indicated times, and western blotting was used to test the protein expression level as indicated. b RAW 264.7-scramble, RAW 264.7-shndrg21#, and RAW 264.7-shndrg22# macrophages were treated with 20 ng/ml TNF-α for the indicated times, followed by western blot analysis. c WT or Ndrg2 knock-in BMDMs were treated with 20 ng/ml TNF-α and subjected to western blot analysis. d WT or Ndrg2−/− BMDMs were treated with or without TNF-α for 4 h. Cytoplasmic and nuclear proteins were separated. Western blotting was used to evaluate p65 nuclear translocation. e WT or Ndrg2 knock-in BMDMs were treated with or without TNF-α for 4 h. Western blotting was used to evaluate p65 expression in the cytoplasm and nucleus. f WT or Ndrg2−/− BMDMs were pretreated with DMSO or BAY-11-7082 for 4 h, followed by treatment with CMT93 cell-conditioned medium for 24 h. M1 and M2 markers were analyzed by q-PCR. n = 3 per group. f WT or Ndrg2−/− BMDMs were pretreated with BAY-11-7082 for 4 h and then induced toward M1, M2, and TAM phenotypes. The culture medium was collected after cultured in serum-free medium for 24 h. IL-12 and IL-10 concentrations were tested via ELISA. n = 3 per group. The results are presented as the mean ± SEM *p < 0.05; **p < 0.01
