Fig. 6: Lenalidomide-induced M2 macrophage polarization is dependent on STAT3.
From: Lenalidomide regulates CNS autoimmunity by promoting M2 macrophages polarization
a Western blotting analysis for expression of STAT3, p-STAT3(Y705), AKT, p-AKT(Y473), STAT6, and p-STAT6 (Y641) in RAW264.7 (upper panel) and BMDMs (lower panel) treated with lenalidomide in different concentration for 4 h (n = 3). b Western blotting analysis for expression of IL10, p-STAT3 (Y705) in RAW264.7 (upper panel), and BMDMs (lower panel) with 25 nM lenalidomide treatment for a gradient time (n = 3). c RAW264.7 (upper panel) and BMDMs (lower panel) were cultured in serum-free medium and treated with lenalidomide for different times. Supernanant was cultured at indicated time to test the concentration of IL10 (n = 3). d RAW264.7 (upper panel) and BMDMs (lower panel) were cultured in serum-free medium, added anti-IL10 antibodies for 12 h and treated with lenalidomide for an additional 4 h. Western blotting analysis for expression of p-STAT3 (Y705) and Ym1. e RAW264.7 (upper panel) and BMDMs (lower panel) were pretreated with CuCu for 2 h and then treated with lenalidomide for an additional 4 h. Western blotting analysis for p-STAT3 (Y705) and Ym1. f Immunostaining of spleen from vehicle- and lenalidomide-treated EAE mice at day 16 using antibodies against p-STAT3 (Y705) (red) and Arg1 (green). Scale bars: 150 μm. GAPDH and β-actin were the loading control
