Fig. 2: Inhibition of SOCCs attenuated PASMC calcification induced by hypoxia. | Cell Death & Disease

Fig. 2: Inhibition of SOCCs attenuated PASMC calcification induced by hypoxia.

From: Granzyme B deficiency promotes osteoblastic differentiation and calcification of vascular smooth muscle cells in hypoxic pulmonary hypertension

Fig. 2

a PASMCs pretreated with verapamil HCl (5 μM), ranolazine 2 HCl (100 μM), 2-APB(10 μM), or HC067047 (10 μM), PASMCs were exposed to hypoxia for 24 h. Expression of Runx2, MSX2, BMP2, SOX9, and SM22α was evaluated with western blotting. β-Actin served as the standard; n = 8. b PASMCs pretreated with verapamil HCl, ranolazine 2 HCl, 2-APB, or HC067047 were cultured under hypoxia for 7 days upon treatment with procalcifying media containing 2.5 mM inorganic phosphate. Vascular calcification was assessed by Alizarin Red S staining b and determination of calcium content c; n = 8. Data are represented as mean ± SEM. *P < 0.05 and **P  < 0.01 versus normoxia group. #P < 0.05, ##P < 0.01, and ###P < 0.001 versus hypoxia group. HC, HC067047; HYP, hypoxia; NOR, normoxia; RAN, ranolazine 2HCl; VERA, verapamil HCl

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