Fig. 3: Expression of GZMB was abnormally decreased in PAH. | Cell Death & Disease

Fig. 3: Expression of GZMB was abnormally decreased in PAH.

From: Granzyme B deficiency promotes osteoblastic differentiation and calcification of vascular smooth muscle cells in hypoxic pulmonary hypertension

Fig. 3

a The cellular location of GZMB was analyzed immunohistochemically, revealing the medial layer as the location of GZMB. All panels are at × 20 magnification except for the zoomed ones. Scale bars = 50 μm; n = 10. b Expression of GZMB was evaluated with western blotting in lung tissues from the normoxia, chronic hypoxia, and MCT-treated rats. β-Actin served as the standard; n = 10. c PASMCs were exposed to hypoxia for 24 h and expression of GZMB was evaluated with western blotting. β-Actin served as the standard; n = 6. d PASMCs were exposed under hypoxia for 24 h and expression of HSPA8 was evaluated with western blotting. β-Actin served as the standard; n = 6. e PASMCs were pretreated with si-HSPA8, expression of GZMB was evaluated with western blotting. β-Actin served as the standard; n = 6. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 versus normoxia group. #P < 0.05, ##P < 0.01, and ###P < 0.001 versus hypoxia + negative control group. CON, control; GZMB, granzyme B; HYP, hypoxia; IP, immunoprecipitation; MCT, monocrotaline; MOCK, transfection reagent; NC, negative control; NOR, normoxia.

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