Fig. 2: NF-κB governed Gomafu expression in vivo and in vitro. | Cell Death & Disease

Fig. 2: NF-κB governed Gomafu expression in vivo and in vitro.

From: Long noncoding RNA Gomafu upregulates Foxo1 expression to promote hepatic insulin resistance by sponging miR-139-5p

Fig. 2

a ChIP-qPCR analysis of the capacity for p65 binding to the Gomafu promoter was performed in hepatocytes from ob/+ and ob/ob mice. Values are means ± s.d. and were representative of three individual experiments. b The ob/ob mice were injected with si-control and sip65 daily for 30 days via the tail vein and Gomafu expression in the liver was determined (n = 7 for each group). Primary mouse hepatocytes were transfected with si-control and sip65 for 24 h and then treated with palmitate (0.4 mmol/l) for another 24 h. c The capacity for p65 binding to the Gomafu promoter and (D) Gomafu expression were measured. e Primary mouse hepatocytes were treated Bay11-7082 (5 μmol/l) for 2 h, then palmitate (0.4 mmol/l) were added for 24 h. Gomafu expression were measured. Values are means ± s.d. and are representative of three individual experiments. **P < 0.01, compared to control. ##P < 0.01, compared to palmitate + siNC or palmitate + Bay

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