Fig. 1: Abnormality of the stem cell pool in the SVZa in Hpx-deficient (Hpx^−/−) mice.

a Representative images of Hoechst-stained coronal sections from brains of wild-type (Hpx^+/+) and Hpx^−/− mice were used to visualize the size of the lateral ventricles. Stereological quantification of the volume of the lateral ventricles was performed. Hpx^−/− brains showed enlarged lateral ventricles. n = 9. b–d The lineage differentiation of NSCs/progenitors was inhibited, and more stem cells remained in an undifferentiated GFAP+ stage. Anti-GFAP (b, green), anti-Dcx (c, red), and anti-MASH1 (d, red) were used to label stem cells/progenitors at different stages in the SVZ in coronal sections of brains. e The number of GFAP+ B cells, MASH1+ C cells, and Dcx+ A cells were measured. GFAP+ stem cells were increased, while Dcx+ and MASH1+ cells were decreased in Hpx^−/− mice. n = 5. *p < 0.05, **p < 0.01. Scale bar: 100 μm in a; 20 μm in b–d. LV lateral ventricle, CC corpus callosum