Fig. 5: Inhibition of SREBP proteolytic processing alters cellular metabolism in colon cancer cells. | Cell Death & Disease

Fig. 5: Inhibition of SREBP proteolytic processing alters cellular metabolism in colon cancer cells.

From: Downregulation of SREBP inhibits tumor growth and initiation by altering cellular metabolism in colon cancer

Fig. 5

Control and fatostatin or 25-HC treated DLD1 cells were subjected to metabolic analysis using the Seahorse XF96 Extracellular Flux analyzer as described in Fig. 4. a The relative levels of OCR associated with basal and maximal respiration, ATP turnover and reserved capacity were calculated based on the measurements obtained in the Mito stress test. Data represent the mean ± SEM (¶p < 0.0001 and §p < 0.01 compared to control untreated cells). b The levels of ECAR associated with glycolysis, glycolytic capacity, and glycolytic reserve were calculated based on the measurements obtained in the glycolysis stress test. Data represent the mean ± SEM (¶p < 0.0001 and §p < 0.01 compared to control untreated cells). c The energetic map of control and treated DLD1 cells was based on the measurements obtained in a and c. The OCR and ECAR data shown in the map represent the OCR of basal respiration from the Mito stress test and the glycolysis-related ECAR from the glycolysis stress test, respectively (data represent the mean ± SEM). d Control and SCAP knockdown Pt130 cells were subjected to metabolic analysis using the Seahorse XF96 Extracellular Flux analyzer. The relative levels of OCR associated with basal and maximal respiration, ATP turnover and reserved capacity were calculated based on the measurements obtained in the Mito stress test. Data represent the mean ± SEM (¶p < 0.0001 compared to Sh-Control). e The levels of ECAR associated with glycolysis, glycolytic capacity and glycolytic reserve was calculated based on the measurements obtained in the glycolysis stress test. Data represent the mean ± SEM (*p < 0.001 and §p < 0.01 compared to Sh-Control). f The energetic map of control and SCAP knockdown Pt130 cells was derived from the measurements obtained in d and e. The OCR and ECAR data shown in the map represent the OCR of basal respiration from the Mito stress test and the glycolysis-related ECAR from the glycolysis stress test, respectively (data represent the mean ± SEM)

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