Fig. 6: Downregulation of SREBP decreases fatty acid oxidation in colon cancer cells.

a, b Knockdown of SREBP1 and SREBP2 reduced fatty acid oxidation (FAO) in DLD1 (a) and Pt130 (b) cells. The rate of cellular FAO was determined by subjecting control and SREBP knockdown cells to the FAO assays using the Seahorse XF96 Extracellular Flux analyzer. Data represent the mean ± SEM (§p < 0.01 compared to Sh-Control). c Knockdown of SCAP reduced FAO in DLD1 and Pt130 cells. The rate of cellular FAO was determined using Seahorse FAO assays. Data represent the mean ± SEM (#p < 0.05, compared to Sh-Control)