Fig. 5: FoxO3A accumulation into the mitochondria only requires the AMPK signal in normal cells and tissues under nutrient shortage. | Cell Death & Disease

Fig. 5: FoxO3A accumulation into the mitochondria only requires the AMPK signal in normal cells and tissues under nutrient shortage.

From: Uncoupling FoxO3A mitochondrial and nuclear functions in cancer cells undergoing metabolic stress and chemotherapy

Fig. 5

a Immunoblot analysis of total proteins isolated from NIH3T3 cells upon LG (0.75 mM glucose, 24 h). β-actin: loading control. b Immunoblot analysis of total and mitochondrial proteins isolated from NIH3T3 cells transfected with the indicated plasmids for 48 h and subjected to LG (24 h). β-actin and HSP60 were used as total lysate and mitochondria controls, respectively. c Left panel: immunoblot analysis of total proteins isolated from kidney and liver of fed or fasted (18 h) mice. β-actin: loading control. Right panel: densitometric analysis of the phosphorylated forms of AMPK and ERK normalized against total AMPK and ERK, respectively, and the loading control. d Left panel: immunoblot analysis of mitochondrial proteins isolated from kidney and liver of fed or fasted (18 h) mice. HSP60: loading control. Right panel: densitometric analysis of full-length and cleaved FoxO3A normalized against the mitochondrial fractionation loading control. e Immunoblot analysis of mitochondrial proteins isolated from the liver of fed or fasted (18 h) mice in the presence or absence of PK. BCL2: outer membrane control, HSP60: mitochondrial matrix control. fl. full-length FoxO3A, cl. cleaved FoxO3A. The presented results are representative of at least three independent experiments. Where applicable, data are presented as mean ± SEM and significance was calculated with Student’s t test; *p < 0.05, **p < 0.01, and ***p < 0.001

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