Fig. 1: Sei-1 overexpression promotes the formation of DMs during in vivo passages | Cell Death & Disease

Fig. 1: Sei-1 overexpression promotes the formation of DMs during in vivo passages

From: Sei-1 promotes double minute chromosomes formation through activation of the PI3K/Akt/BRCA1-Abraxas pathway and induces double-strand breaks in NIH-3T3 fibroblasts

Fig. 1

. a Metaphase spread images of Vec, CPX1, and CPX6 samples. Red arrows represent the DMs. b Quantification of DMs in Vec, CPX1, and CPX6 samples; n = 100 from three independent experiments for each group. c Quantitative real-time PCR results of Sei-1 relative mRNA expression, n = 3. d Western blot results show increases in Sei-1 expression from the Vec, CPX1, and CPX6 samples during in vivo passages. e Quantification of the protein levels in d, n = 3. *P < 0.05; **P < 0.01; ***P < 0.001

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