Fig. 5: Inhibition of the PI3K/Akt/BRCA1-A complex pathway can result in the loss of DMs | Cell Death & Disease

Fig. 5: Inhibition of the PI3K/Akt/BRCA1-A complex pathway can result in the loss of DMs

From: Sei-1 promotes double minute chromosomes formation through activation of the PI3K/Akt/BRCA1-Abraxas pathway and induces double-strand breaks in NIH-3T3 fibroblasts

Fig. 5

. a, b Western blot results indicate effective inhibition of the Akt pathway with the MK2206 inhibitor at 10 µM and increases of the downstream BRCA1-A complex in CPX1 or CPX6. Quantification of the protein levels, n = 3. c Metaphase spread images of CPX6 after inhibition of the Akt pathway. Red arrows indicate DMs. d Quantification of DMs in C, n = 100 from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001

Back to article page