Fig. 3: MSCs alleviated DNA damage and ROS accumulation of liver cells induced by DEN.
From: The distinct roles of mesenchymal stem cells in the initial and progressive stage of hepatocarcinoma
a Immunostaining for γ-H2AX in the rat livers of DEN and DEN + MSC (Is) at 8 weeks after DEN administration. Number of positive cells was shown in the graphs (right panel). b Livers of rats in DEN and DEN + MSC (Is) group were removed and cell lysates were immunoblot with the indicated antibodies at 8th week. c, d Lipid peroxidation and oxidation resistance was examined at 8th week by measuring MDA c and T-AOC d in liver homogenate, respectively. e Liver cryosections of rats in DEN and DEN + MSC (Is) group were incubated with 2 μm MitoSOX Red mitochondrial superoxide indicator for 30 min. Staining positive cells were identified by fluorescence microscopy (left panel) and quantification is shown at right. All scale bars = 100 μm. Values are shown as mean ± SEM (**P < 0.01, ***P < 0.001). Representative of three independent experiments
