Fig. 5: Administered MSCs promotes tumor cell proliferation in the Ps of hepatocarcinoma progression.
From: The distinct roles of mesenchymal stem cells in the initial and progressive stage of hepatocarcinoma
a, b Immunostaining for Ki67 a and Tunel staining b in the liver sections in DEN and DEN + MSC (Ps) group at 14 weeks after DEN treatment. Dashed lines indicate the tumor area. The semiquantitative analysis was shown in the graph (right panel). Scale bar: 100 μm. c At 14th week, livers in DEN and DEN + MSC (Is) group were removed and lysed to assess the expression of CyclinD1, Puma, and Bim by immunoblot with indicated antibodies. d, e Rat CBRH-7919 cells were treated with conditioned medium of MSC (MSC-CM) or MSC pretreated by 10 ng/ml TNF-α/IFN-γ for 12 h (MSC (IF)-CM). The cell proliferation was assayed by CCk8 at different times d and the influence of conditional medium on 7919 cells proliferation was compared at 72 h e. f, g Cell cycle analysis of 7919 cells was measured by flow cytometry e and the fraction of S + G2 phase was shown g. Values are shown as mean ± SEM (n = 3, *P < 0.05, **P < 0.01). Representative of four independent experiments
