Fig. 7: Administered MSCs promoted EMT of liver cancer cell in the Ps of hepatocarcinoma development.
From: The distinct roles of mesenchymal stem cells in the initial and progressive stage of hepatocarcinoma
a Immunostaining for E-cadherin and Vimentin in the rat livers of DEN and DEN + MSC (Ps) group. The positive staining was quantified by Image-Pro Plus software (right panel) (bar: 100 μm). b The wound-healing assay was employed to determine the migration of CBRH-7919 cells exposure with DMEM medium (Cont), MSC-CM or MSC (IF)-CM for 2 days and percentage of migration area was quantified as shown in right panel. c Invasiveness of cells was determined using the Transwell assay. CBRH-7919 cells were plated in the upper chamber without FBS for 24 h, and MSC-CM or MSC (IF)-CM with 10% FBS was placed in the lower chamber. The number of cells that invaded through the Matrigel was counted in 10 fields under the ×20 objective lens. d Immunofluorescence staining was used to detect the expression of E-cadherin and Vimentin in CBRH-7919 cells (bar: 100 μm). Values are shown as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001). Representative of four independent experiments
