Fig. 5: The cytoprotective effect of TSA is abrogated in Atg7-KO mouse kidney proximal tubular cells. | Cell Death & Disease

Fig. 5: The cytoprotective effect of TSA is abrogated in Atg7-KO mouse kidney proximal tubular cells.

From: Histone deacetylase inhibitors protect against cisplatin-induced acute kidney injury by activating autophagy in proximal tubular cells

Fig. 5

Atg7 WT and Atg7 KO MPTC cells were untreated or treated with cisplatin (20 μM), TSA (0.1 μM), or cisplatin+TSA for 20 h. After treatment, cells were collected for morphological and biochemical analysis. a Representative images of cellular and nuclear morphology of apoptosis (×200). Scale bar: 200 μm. b Quantification of apoptosis percentage. c Efficiency of apoptosis inhibition by TSA (Atg7-WT vs Atg7-KO). d Caspase activity measured by enzymatic assays. e Efficiency of TSA reducing caspase activation (cisplatin only vs cisplatin+TSA). Data in b–e are expressed as mean ± SD. *P < 0.05, significantly different from the control group. #P < 0.05, significantly different from the cisplatin-only group. ^P < 0.05, significantly different from the corresponding group in Atg7 WT cells

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