Fig. 7: Chloroquine abolishes the protective effects of TSA in cisplatin-induced AKI in mice. | Cell Death & Disease

Fig. 7: Chloroquine abolishes the protective effects of TSA in cisplatin-induced AKI in mice.

From: Histone deacetylase inhibitors protect against cisplatin-induced acute kidney injury by activating autophagy in proximal tubular cells

Fig. 7

C57BL/6 mice were divided into five groups for the following treatment: (1) saline (n = 8), (2) a single dose of cisplatin (30 mg/kg, i.p.) only (n = 12), (3) cisplatin+TSA (1 mg/kg, i.p., daily) (n = 15), (4) cisplatin+chloroquine (60 mg/kg, i.p., daily) (n = 6), and (5) cisplatin+TSA+chloroquine (n = 7). Blood samples and kidneys were collected 3 days after treatment for biochemical and histological analyses. a Serum creatinine. b Representative images of kidney H–E staining (×200). Scale bar: 100 μm. c Pathological score of tubular damage. Data in a and c are expressed as mean ± SD. *P < 0.05, significantly different from the saline group. #P < 0.05, significantly different from the cisplatin-only group. &P<   0.05, significantly different from the cisplatin+TSA group. d Representative immunoblots of cleaved caspase 3. e Densitometric analysis of cleaved caspase 3 signals. Data are expressed as mean ± SD. *P < 0.05, significantly different from the saline group. #P < 0.05, significantly different from the cisplatin-only group. &P < 0.05, significantly different from the cisplatin with the TSA group

Back to article page