Fig. 9: The renoprotective effect of TSA in cisplatin-induced AKI is lost in PT-Atg7 KO mice. | Cell Death & Disease

Fig. 9: The renoprotective effect of TSA in cisplatin-induced AKI is lost in PT-Atg7 KO mice.

From: Histone deacetylase inhibitors protect against cisplatin-induced acute kidney injury by activating autophagy in proximal tubular cells

Fig. 9

PT-Atg7 WT and PT-Atg7 KO mice were injected with saline or a single dose of cisplatin (30 mg/kg, i.p.) in the absence (−) or presence (+) of TSA (1 mg/kg, i.p., daily injection). Three days after treatment, blood samples and kidneys were collected for biochemical and histological analyses. a Serum creatinine. b Efficiency of TSA reducing serum creatinine (cisplatin only vs cisplatin+TSA). c BUN. d Efficiency of TSA reducing BUN (cisplatin only vs cisplatin+TSA). Data in a–d are expressed as mean ± SD. *P < 0.05, significantly different from the saline group. #P < 0.05, significantly different from the cisplatin-only group. ^P < 0.05, significantly different from the corresponding group of PT-Atg7 WT mice. e Representative images of kidney H–E staining (×200). Scale bar: 100 μm. f Pathological score of tubular damage. Data are expressed as mean ± SD. #P < 0.05, significantly different from the cisplatinonly group. ^P < 0.05, significantly different from the PT-Atg7-WT group. g Representative immunoblots. h Densitometric analysis of cleaved caspase 3 signals. Data are expressed as mean ± SD. *P < 0.05, significantly different from the saline group. #P < 0.05, significantly different from the cisplatin-only group. ^P < 0.05, significantly different from the PT-Atg7-WT group

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