Fig. 4: Novel peptide-based inhibitors of cathepsin G suppress IL-36β activation by proteases contained within neutrophil degranulates.
From: Suppressing IL-36-driven inflammation using peptide pseudosubstrates for neutrophil proteases
a–c HeLaIL-36R-SEAP cells were either left untreated, or were stimulated either with recombinant IL-36α, IL-36β, or IL-36γ (500 pM) that had been pre-incubated for 2 h at 37 °C, either alone, with unstimulated neutrophil supernatant (Ctrl s/n), or PMA-activated human neutrophil supernatant (PMA s/n) in the presence or absence of a titration (10, 5, 2.5, 1, 0.5, or 0.25 μM) of candidate peptide inhibitors. After 24 h, cytokine concentrations in the culture supernatants were determined by ELISA. Results shown are representative of at least three independent experiments. Error bars represent the mean ±SEM of triplicate determinations from a representative experiment. Asterisk(s) indicate significance levels, ***p < .0001, **p < .001, *p < .1, by Student’s t test
