Fig. 5: Time course analysis of inhibition of IL-36β activation by cathepsin G-specific peptide antagonists.
From: Suppressing IL-36-driven inflammation using peptide pseudosubstrates for neutrophil proteases
Cathepsin G was pre-incubated for 30 min on ice in the presence or absence of the indicated cathepsin G peptide inhibitors (at 10 μM) followed by incubation at 37 °C with recombinant IL-36β (500 pM) for 20–120 min as indicated. HeLaIL36R-SEAP cells were then incubated with samples of each reaction taken at the indicated time points. After 24 h, cytokine concentrations in cell culture supernatants were determined by ELISA. Results shown are representative of at least three independent experiments. Asterisk(s) indicate significance levels, ***p < .0001, **p < .001, *p < .1, by Student’s t test
