Fig. 8: Bi-specific inhibitors of cathepsin G and elastase block IL-36β and IL-36γ activation.
From: Suppressing IL-36-driven inflammation using peptide pseudosubstrates for neutrophil proteases
a,b HeLaIL-36R-SEAP cells were stimulated with a IL-36β (500 pM) or b IL-36γ (500 pM), pre-incubated for 2 h at 37 °C with a fixed dose (1:4) of PMA-activated neutrophil degranulate (or control unstimulated neutrophil S/N) in the presence or absence of a titration (10, 5, 2.5, 1, 0.5, or 0.25 μM) of candidate peptide inhibitors. After 24 h, cytokine concentrations in the culture supernatants were determined by ELISA. Results shown are representative of at least three independent experiments. Error bars represent the mean ±SEM of triplicate determinations from a representative experiment
