Fig. 5: NF-κB activation promotes survival of ccRCC cells treated with sunitinib. | Cell Death & Disease

Fig. 5: NF-κB activation promotes survival of ccRCC cells treated with sunitinib.

From: The convergent roles of NF-κB and ER stress in sunitinib-mediated expression of pro-tumorigenic cytokines and refractory phenotype in renal cell carcinoma

Fig. 5

a Wild type (WT) 786-O cells, IKKβ knockout subline and cells expressing HA-tagged dominant-negative mutant IκBα (S32A/S36A) were treated with 10 μM of sunitinib for 24 h. Apoptosis was examined using APO-BRDU kit followed by flow cytometry analysis. Data are presented as the mean ± S.D. *P = 0.0002; **P < 0.0001; ***P < 0.0001 when compared with WT 786-O cells treated with sunitinib. b 786-O cells were treated with either 10 μM of sunitinib (Sun) or 10 μM of sorafenib (Sor) for 24 h with or without IKKβ inhibitor, IKK-16 (5 μM). Apoptosis was examined using APO-BRDU kit followed by flow cytometry analysis. Data are presented as the mean ± S.D. ***P < 0.0001.

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