Fig. 4: Overexpression of PARK2 inhibits osteosarcoma growth in vivo.

The photograph shows tibial tumor formation in two groups (a). Compared with the NC group, PARK2 significantly decreased tumor size and tumor weight by the end of the experiment (b, c). PARK2 expression in tibial xenografts was confirmed by immunohistochemistry (IHC) (d1–2). Histological characteristics were evaluated by H&E staining; extensive tumor cell necrosis, numerous hematocytes, and vascular structures (black arrows) were observed in the NC group (d3–6). Joint structure destruction was evaluated by Masson staining (d7–10). The western blot assay showed the expression level of the proteins VEGF and PARK2 in tibial xenografts in two groups that the expression of the protein VEGF was negatively correlated with PARK2 (e). Magnification, ×20 (d3–4), ×40 (d7–8), ×100 (d9–10), ×400 (d1–2, 5–6). Scale bar, 1000 μm (d3–4), 500 μm (d7–8), 200 μm (d9–10), 50 μm (d1–2, 5–6). *P < 0.05, **P < 0.01, ***P < 0.001