Fig. 7: Overexpression of PARK2 decreases angiogenesis in vivo.
From: PARK2 inhibits osteosarcoma cell growth through the JAK2/STAT3/VEGF signaling pathway
Nude mice were injected on both sides of the groin subcutaneously with Matrigel mixed with the stable transfected HOS cells (8 × 106 cells; PARK2 on the right side; NC on the left side) and were left for 7 days. General morphology and color of the Matrigel plugs were recorded and plugs were quantified for hemoglobin content (a). Hematocytes and vascular structures (black arrows) were detected by H&E staining (b). Vascular endothelial-like structures were examined by a CD34 immunofluorescence staining assay (c). Magnification, ×40 ((b1, 3), (c1–3, 5–7)), ×400 ((b2, 4), (c4, 8)). Scale bar, 500 μm ((b1, 3), (c1–3, 5–7)) and 50 μm ((b2, 4), (c4, 8)). *P < 0.05, **P < 0.01, ***P < 0.001
