Fig. 6: NRBP1 promotes apoptosis through the activation of JNK in CRC cells. | Cell Death & Disease

Fig. 6: NRBP1 promotes apoptosis through the activation of JNK in CRC cells.

From: Nuclear receptor binding protein 1 correlates with better prognosis and induces caspase-dependent intrinsic apoptosis through the JNK signalling pathway in colorectal cancer

Fig. 6

a The effects of NRBP1 overexpression on targets in the JNK, P38, ERK1/2 signalling pathways were assessed by western blot in SW480 and HCT116 cells. GAPDH was used as loading control. b The effects of NRBP1 silencing on targets in the JNK, P38, ERK1/2 signalling pathways were assessed by western blot in RKO and DLD1 cells. GAPDH was used as loading control. c SW480 and HCT116 cells were treated with SP600125 for 30 min. Twenty-four hours later, caspase-related proteins were evaluated by western blot. c Apoptotic ratios were analysed by double staining the cells with Annexin V and PI using flow cytometry. Representative images from triplicate experiments are shown (top). Q3-1 necrosis cells. Q3-2 late apoptosis cells. Q3-3 normal cells. Q3-4 early apoptosis cells. Graphs (bottom) show quantitative analysis of total apoptosis (*P < 0.05, **P < 0.01)

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