Fig. 1: Rb is required for the development of intraretinal vascular plexi and the OPL.
From: Rb is required for retinal angiogenesis and lamination
a Isolectin B4 (IB4) staining of P18 whole-mount retinas of Rbf/f (WT control) and Rbf/f;Z/Red;a-Cre (RbKO in red areas) mouse. Selected areas are blown up to show the vascular density. b Whole-mount retinas of the indicated ages and genotypes were stained for IB4. c Confocal images of IB4-stained SVP, IVP, and DVP of P18 whole-mount retinas of the indicated genotypes. Pseudo-colors were used to differentiate these three plexi. d IB4 (green) and DAPI (blue) staining of P18 retinal sections of WT or RbKO retina. e Horizontal sections of indicated ages and genotypes were stained for nuclear (DAPI, blue), mitosis (PH3, green at P0, red at P8). Arrows in d and e indicate the position of outer plexiform layer (OPL). The dotted lines in a–c indicate the boundary between WT (in the center) and RbKO areas (in the periphery). NBL neuroblast layer, ONL outer nuclear layer, INL inner nuclear layer, IPL inner plexiform layer, GCL ganglion cell layer. Scale bar is 50 µm
