Fig. 3: E2f1 mediates the RbKO-induced retinal angiogenesis and lamination defects.
From: Rb is required for retinal angiogenesis and lamination
a P18 retinal sections of the indicated genotypes were stained for nuclear (DAPI, blue), vascular endothelium cells (IB4, green), and amacrine cells (Calretinin, red). White arrows indicate Calretinin+ tracks in the IPL; loss of the outer two tracks reveals the starburst amacrine cell defect. b P18 whole-mount retinas of indicated genotypes were stained for IB4 to label vasculature. Dotted lines indicate the boundary between WT (center) and RbKO (peripheral) areas. c Confocal images of IB4-stained SVP, IVP, and DVP of P18 whole-mount retinas of indicated genotypes. Pseudo-colors were used to differentiate these three plexi. d–f Quantification of vessel coverage (d), average vessel length (e), and lacunarity (f) by the AngioTool software. Error bars represent SD of measurements from at least three animals and asterisks indicate significant differences between retinas of RbKO and the indicated genotypes (*p < 0.05, **p < 0.01, one-way ANOVA followed by Bonferroni correction). Scale bar is 50 μm. ONL outer nuclear layer, INL inner nuclear layer, GCL ganglion cell layer, ON optic head
