Fig. 5: BaxKO ameliorates the vascular defect in the RbKO retina.
From: Rb is required for retinal angiogenesis and lamination
a Isolectin B4 (IB4) staining of P18 whole-mount retinas of Rbf/f;Bax−/−;a-Cre mouse. b P18 retinal sections of indicated genotypes were stained for nuclei (DAPI, blue), vascular endothelial cells (IB4, green), and amacrine cells (Calretinin, red). Arrows highlight the amacrine neurite tracks in the IPL. ONL outer nuclear layer, INL inner nuclear layer, GCL ganglion cell layer. c Confocal images of IB4-stained superficial, intermediate, and deep plexi of P18 whole-mount retinas of indicated genotypes. Pseudo-colors were used to differentiate the three plexi. d–f Quantification of vessel coverage (d), average vessel length (e), and lacunarity (f) by the AngioTool software. Error bars represent SD of measurements from at least three animals and asterisks indicate significant difference between WT and other genotypes, or between RbKO and Rb/Bax DKO as indicated by square brackets (*p < 0.05, one-way ANOVA followed by Bonferroni correction). Scale bar in b and c is 50 µm
