Fig. 6: Lamination defects in the Rb/Bax DKO retina.
From: Rb is required for retinal angiogenesis and lamination
a Retinal sections of indicated genotypes and ages were stained for nuclei (DAPI, blue), synaptic vesicles (SV2a, red; Syt1, green), cones (M opsin, red), and horizontal cells (OC2, blue). b RT-PCR analysis of the indicated genes and ages (n = 3). c Retinal sections of indicated genotypes and ages were stained for nuclei (DAPI, blue), division (Ki67, green), and synaptic vesicles (SV2a, red). Arrows indicate ectopically dividing cells disrupting the SV2+ OPL. d Retinal sections of indicated genotypes and ages were stained for nuclei (DAPI, blue), cyclin E (red), Cdk5 (red), and Dcx (red). e P18 Rb/Bax DKO retinal sections were stained for nuclei (DAPI, blue), cone (cone arrestin, red), horizontal (D28K, green), Müller (GS, green), or amacrine cells (Calretinin, red; Ap2a, red). Arrowheads highlight the OMPL separating the INL. f Retinal thickness for the indicated genotypes and ages. g Cell counts of cone (cone arrestin+), horizontal (D28K+), amacrine (Ap2a+), and Müller cells (GS+) of the indicated genotypes at P18. h P18 Rb/Bax DKO retinal sections were stained for nuclei (DAPI, blue), division (Ki67, green), and amacrine cells (Calretinin, red). Dendrites of Calretinin+ amacrine cell stratify in the IPL, as usual, but also the OMPL (arrow). Data are mean ± SD. Asterisks indicate significant difference between WT and other genotypes, or between RbKO and Rb/Bax DKO as indicated by square brackets (*p < 0.05, one-way ANOVA followed by Bonferroni correction). ONL outer nuclear layer, OMPL outer misplaced plexiform layer, OPL outer plexiform layer, INL inner nuclear layer, IPL inner plexiform layer, GCL ganglion cell layer. Scale bar is 50 µm
