Fig. 1: BKM120 affects Akt/mTOR signaling and has anti-proliferative as well as pro-apoptotic activity. | Cell Death & Disease

Fig. 1: BKM120 affects Akt/mTOR signaling and has anti-proliferative as well as pro-apoptotic activity.

From: Pan-class I  PI3-kinase inhibitor BKM120 induces MEK1/2-dependent mitotic catastrophe in non-Hodgkin lymphoma leading to apoptosis or polyploidy determined by Bax/Bak and p53

Fig. 1

a Phosphorylation of distinct PI3K downstream targets in MINO, GRANTA-519, and SU-DHL-10 after incubation with BKM120 at the indicated concentrations for 6 h. b Panel of non-Hodgkin lymphoma cell lines incubated with increasing concentrations of BKM120 for 72 h. Metabolic activity was assessed using the XTT assay (upper panel). Cell death induction was assessed using PI uptake (middle panel). Time dependent proliferation was assessed by counting the cell number of NHL cells treated with 2 µM and exclusion of dead cells by trypan blue staining (lower panel). All panels show mean and SD of three independent experiments. c Annexin-V-FITC/PI assay of the B-NHL cell line panel after incubation with 2 µM BKM120 for 72 h. Percentages refer to cells positive for both, PI and Annexin-V-FITC staining (upper right quadrant) and cells positive only for Annexin-V-FITC staining (lower right quadrant), respectively. Results are representative for three independent experiments

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