Fig. 2: BKM120 kills cells via the intrinsic apoptosis pathway. | Cell Death & Disease

Fig. 2: BKM120 kills cells via the intrinsic apoptosis pathway.

From: Pan-class I  PI3-kinase inhibitor BKM120 induces MEK1/2-dependent mitotic catastrophe in non-Hodgkin lymphoma leading to apoptosis or polyploidy determined by Bax/Bak and p53

Fig. 2

a Annexin-V-FITC/PI assay in MINO and JEKO-1 cells in response to 2 µM BKM120 for 48 h and concomitant blockage of caspases with 10 µM QV-D-OPh. Percentages refer to cells positive for both, PI and Annexin-V-FITC staining (upper right quadrant) and cells positive only for Annexin-V-FITC staining (lower right quadrant), respectively. Results are representative for three independent experiments. b Measurement of the protein levels of Bax and Bak and cleavage/activation of caspase-9 in MINO cells in response to 1 µM and 1.5 µM of BKM120 incubated for 24 h. c Relative mRNA expression levels of Bcl-2 family members after incubation of JEKO-1 cells with 1.5 µM of BKM120 for the indicated time points [h]. Mean ± SD of three independent experiments

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