Fig. 5: Concomitant Bax, Bax, and p53 deficiency determines polyploidy. | Cell Death & Disease

Fig. 5: Concomitant Bax, Bax, and p53 deficiency determines polyploidy.

From: Pan-class I  PI3-kinase inhibitor BKM120 induces MEK1/2-dependent mitotic catastrophe in non-Hodgkin lymphoma leading to apoptosis or polyploidy determined by Bax/Bak and p53

Fig. 5

a Basal protein expression levels of apoptotic regulators in a panel of NHL cell lines. b Annexin-V-FITC/PI assay of HCT116 WT or HCT116 Bax−/−/Bak−/− cells incubated with increasing concentrations of BKM120 for 72 h (upper panel). Annexin-V-FITC/PI assay of HCT116 WT or HCT116 Bax−/−/Bak−/− cells co-transfected with ctrl or p53 siRNA and incubated with 2.5 µM BKM120 for 72 h (lower panel). Data show mean ± SD of three independent experiments. c Polyploidy assay of HCT116 WT or HCT116 Bax−/−/Bak−/− cells co-transfected with ctrl or p53 siRNA and incubated with increasing concentrations of BKM120 for 72 h. Data show mean ± SD of three independent experiments. *p < 0.05; **p < 0.005

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