Fig. 7: RA inhibits the proliferation and invasion of MDA-MB-231 cells through promotion of apoptosis and inhibition of AKT/mTOR signaling pathways. | Cell Death & Disease

Fig. 7: RA inhibits the proliferation and invasion of MDA-MB-231 cells through promotion of apoptosis and inhibition of AKT/mTOR signaling pathways.

From: Raddeanin A suppresses breast cancer-associated osteolysis through inhibiting osteoclasts and breast cancer cells

Fig. 7

a Cell viability of RA-treated MDA-MB-231 cells tested by CCK-8 assays at 48 and 96 h. b MDA-MB-231 cells were treated with various concentrations of RA for 24 h and then evaluated with EdU incorporation assay (n = 3 per group). Magnifications: ×100. c The percentages of EdU-positive cells for each field. d MDA-MB-231 cells were treated with various doses of RA for 48 h and then stained with Annexin V and propidium iodide for flow cytometric analysis (n = 3 per group). e Apoptotic rate was defined as the percentage of dead and apoptotic cells (quandrants 2 and 3). f RA inhibited the invasion of MDA-MB-231 cells by Transwell invasion assay (n = 3 per group). Magnifications: ×200. g The number of invaded cells of each field was counted. h MDA-MB-231 cells were treated with or without RA (3 μM) for 0, 6, or 12 h, respectively. Western blotting for p-AKT and mTOR was analyzed with the cell lysates (*p < 0.05, **p < 0.01)

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