Fig. 4: The phosphorylation of eIF2α protects cells by a mechanism that involves adaptive autophagy. | Cell Death & Disease

Fig. 4: The phosphorylation of eIF2α protects cells by a mechanism that involves adaptive autophagy.

From: Inhibition of Sec61-dependent translocation by mycolactone uncouples the integrated stress response from ER stress, driving cytotoxicity via translational activation of ATF4

Fig. 4

a HeLa-gs cells stably expressing GFP-GADD34 (clone 8) or GFP-KARA (clone 4), an inactive mutant of GADD34, were treated with mycolactone for 4 days The number of apoptotic cells (positive for both active caspase 3/7 and PI) were determined for three fields and expressed as a proportion of total cells (Mean ± SEM n = 4 independent experiments). b Wild-type and PERK−/− GCN2−/− MEFs were treated with mycolactone for 24 h and analysed by confocal microscopy as in (a). c, d HeLa cells were treated as shown or with chloroquine (CQ) for 12 h. Equal protein quantities in lysates were analysed by immunoblotting. e WT and PERK−/− GCN2−/− MEFs were treated as in (c). Lysates were analysed by immunoblotting. All data representative of at least three independent experiments. All immunoblots show the approximate migration of molecular weight markers in kDa. See also Fig. S4

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